Sanger Method

Fred Sanger developed this technology in the 1980s after the invention of PCR made sequencing possible. This method uses 4 flourescent dyes that bind to each of the bases. The machine is able to piece together the sequence by stopping at the same type of base (GATC) everytime it appears and piecing together the sequence. Visit the page to learn more about this method.

2nd Generation Sequencing

Illumina

Second Generation sequencing is significantly more efficient than it's sanger method counterpart. It is able to process high throughput sequencing jobs and uses a different method. Learn more about second generation sequencing in the page.

3rd Generation Sequencing

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3rd generation sequencing is even more efficient and cost efficient than 2nd generation sequencing. However, because it is such a new technology, it has not been widely employed in the field. Third generation technology also negates the use of PCR and outrules PCR bias.